Membrane Mimic

Membrane Mimic


Two Apposing Monolayers Form a Droplet Interface Bilayer (DIB): A Model Cell Membrane. Self-assembled structure of microdroplet at the monolayer and bilayer provides unique opportunities to study the intriguing chemistry at the liquid-liquid interface.

Lipid membranes represent the most important biological interface. The structure and properties of lipid membranes are strongly modulated by the composition of constituent lipids and the surrounding ionic aqueous medium. Our interests include how and to what extent do various compositions of lipids and salts affect the structure and property of lipid bilayers, using droplet interface bilayer as a model membrane. In our recent study, we demonstrated that the droplet interface bilayer (DIB) can be employed as a convenient model biological membrane to rapidly explore subtle structural effects on bilayer water permeability.

For more details, please visit our recent publications. (undergraduate co-authors are underlined)

  • Michael Krmic, Escarlin Perez, Patrick Scollan, Katherine Ivanchenko, Alondra Gamez Hernandez, Joseph Giancaspro, Juan Rosario, Jasmin Ceja-Vega, Jamie Gudyka, Riley Porteus, and Sunghee Lee, Aspirin Interacts with Cholesterol-Containing Membranes in a pH-Dependent Manner, Langmuir, November 8, 2023. https://pubs.acs.org/doi/10.1021/acs.langmuir.3c02242
  • Jasmin Ceja-Vega, Escarlin Perez, Patrick Scollan, Juan Rosario, Alondra Gamez Hernandez, Katherine Ivanchenko, Jamie Gudyka, and Sunghee Lee,Resveratrol decreases membrane water permeability: a study of cholesterol-dependent interactions, The Journal of Membrane Biology, 1–16, 2022 https://link.springer.com/article/10.1007/s00232-022-00250-0
  • Escarlin Perez, Jasmin Ceja-Vega,Krmic, Michael, Alondra Gamez Hernandez, Jamie GudykaRiley Porteus,Sunghee Lee, Differential Interaction of Cannabidiol with Biomembranes Dependent on Cholesterol Concentration, ACS Chem. Neuroscience, 2022, 13 (7), 1046–1054  https://pubs.acs.org/doi/10.1021/acschemneuro.2c00040
  • Giancaspro J, Scollan P, Rosario J, Miller E, Braziel S, Lee S. Structural determination of model phospholipid membranes by Raman spectroscopy: Laboratory experiment. Biochem Mol Biol Educ. 2022; 1–12. https://doi.org/10.1002/bmb.21603
  • Megan Wood, Michael Morales, Elizabeth Miller, Samuel Braziel, Joseph Giancaspro, Patrick Scollan, Juan Rosario, Alyssa Gayapa, Michael Krmic, and Sunghee Lee, "Ibuprofen and the Phosphatidylcholine Bilayer: Membrane Water Permeability in the Presence and Absence of Cholesterol" Langmuir, 2021, 37(15), pp.4468-4480. https://pubs.acs.org/doi/10.1021/acs.langmuir.0c03638
  • Shea Foley, Elizabeth Miller, Samuel Braziel, and Sunghee Lee, “Molecular Organization in Mixed SOPC and SDPC Model Membranes: Water Permeability Studies of Polyunsaturated Lipid Bilayers”, Biochimica et Biophysica Acta (BBA)-Biomembranes, 1862 (2020) 183365. https://doi.org/10.1016/j.bbamem.2020.183365
  • Lee, Sunghee, Good to the Last Drop: Interfacial Droplet Chemistry, from Crystals to Biological Membranes, Acc. Chem. Res., 2018, 51 (10), 2524–2534. https://pubs.acs.org/doi/10.1021/acs.accounts.8b00277
  • Lopez, Maria; Denver, Jacqueline; Evangelista, Sue Ellen; Armetta, Alessandra; Di Domizio, Gabriella; Lee, Sunghee “Effects of Acyl Chain Unsaturation on Activation Energy of Water Permeability Across Droplet Bilayers of Homologous Monoglycerides: Role of Cholesterol” Langmuir, 2018, 34(5), 2147-2157. http://dx.doi.org/10.1021/acs.langmuir.7b03590
  • Samuel Braziel, Kalen Sullivan, and Sunghee Lee. “Quantitative Raman microspectroscopy for water permeability parameters at a droplet interface bilayer.” Analyst, 2018, 143(3), 747-755. http://dx.doi.org/10.1039/c7an01349c
  • Maria Lopez, Sue Ellen Evangelista, Melissa Morales, and Sunghee Lee, “Enthalpic Effects of Chain Length and Unsaturation on Water Permeability across Droplet Bilayers of Homologous Monoglycerides”, Langmuir, 2017, 33 (4), pp 900–912. http://dx.doi.org/10.1021/acs.langmuir.6b03932
  • P.J. Milianta, M. Muzzio, J. Denver, G. Cawley, S.Lee, “Water Permeability Across Symmetric and Asymmetric Droplet Interface Bilayers: Interaction of Cholesterol Sulfate with DPhPC”, Langmuir, 31 (44), pp 12187–12196, 2015, http://dx.doi.org/10.1021/acs.langmuir.5b02748
  • Z. Michalak, M. Muzzio, P.J. Milianta, R. Giacomini, S.Lee, “Effect of Monoglyceride Structure and Cholesterol Content on Water Permeability of the Droplet Bilayer”, Langmuir, 29 (51), 15919-15925, 2013. http://pubs.acs.org/doi/abs/10.1021/la4040535
  • Z. Michalak, D. Fartash, N. Haque, and S.Lee, “Tunable Crystallization via Osmosis-Driven Transport Across a Droplet Interface Bilayer”, CrystEngComm., 14, 7865-7868, 2012. http://dx.doi.org/10.1039/C2CE26249E
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